GC-MS Based Metabolomic Profiling of Streptomyces clavuligerus Isolated from Ocimum gratissimum Rhizosphere

Streptomyces clavuligerus is a member of the Actinobacteria family primarily known for its production clavulanic acid antibiotic. The need identification new antimicrobials led to volatile components S. metabolites using GC-MS. isolate was obtained from Ocimum gratissimum rhizosphere starch casein agar, and identified molecular typing. preliminary antibacterial screening carried out some indicator bacteria wound sites urinary tract infection. Its bioactive were sub-merged fermentation over four-day period, compounds organism showed significant (p<0.05) inhibition on Pseudomonas aeruginosa Escherichia coli. Metabolomics study revealed presence alkanone alkene functional groups. Eicosene major antimicrobial compound identified. Likewise, non-antimicrobial, steroidal metabolite – pregnelonone, also dominant in mixture produced by organism. Identifying constituents microbial may be route obtaining GC-MS useful tool achieving such aim.